The issue of plasma asymmetric dimethylarginine reference range – A systematic review and meta-analysisReport as inadecuate

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Asymmetric dimethylarginine ADMA is an endogenous inhibitor of nitric oxide synthase, marker and mediator of endothelial dysfunction. Several studies have demonstrated its value in cardiovascular risk stratification and all-cause mortality prediction. The aim was to determine the reference range of plasma ADMA in healthy adults.

Methods and results

Taking into account the most widely used ADMA measurement methods, only studies using either high performance liquid chromatography HPLC -with fluorescence or mass spectrometric detection-, or enzyme-linked immunosorbent assay ELISA to quantify plasma ADMA concentrations were enrolled. 66 studies were included in the quantitative analysis 24 using ELISA and 42 using HPLC reporting a total number of 5528 non-diabetic, non-hypertensive, non-obese adults without any medication 3178 men and 2350 women, 41.6 ± 16.9 years old. The reference range of ADMA in μmol-l with 95% confidence interval in parenthesis was 0.34 0.29–0.38– 1.10 0.85–1.35 with a mean of 0.71 0.57–0.85 n = 4093 measured by HPLC and 0.25 0.18–0.31– 0.92 0.76–1.09 with a mean of 0.57 0.48–0.66 n = 1435 by ELISA.


Numerous publications suggested that asymmetric dimethylarginine is not only an outstanding tool of disease outcome prediction but also a new potential therapeutic target substance; the reference range provided by this meta-analysis can become of great importance and aid to further investigations. However, developing a standard measurement method would be beneficial to facilitate the clinical usage of ADMA.

Author: Balázs Németh , Zénó Ajtay, László Hejjel, Tamás Ferenci, Zoltán Ábrám, Edit Murányi, István Kiss



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