Metabolites contributing to Rhizoctonia solani AG-1-IA maturation and sclerotial differentiation revealed by UPLC-QTOF-MS metabolomicsReport as inadecuate




Metabolites contributing to Rhizoctonia solani AG-1-IA maturation and sclerotial differentiation revealed by UPLC-QTOF-MS metabolomics - Download this document for free, or read online. Document in PDF available to download.

Rhizoctonia solani is a causative agent of sheath blight, which results in huge economic losses every year. During its life cycle, the formation of sclerotia helps Rhizoctonia solani withstand a variety of unfavorable factors. Oxidative stress is a key factor that induces sclerotium formation. The differentiated and undifferentiated phenotypes of R. solani AG-1-IA were obtained by controlling aerial conditions. Metabolomics based on the mass spectrometry technique combined with multivariate and univariate analyses was used to investigate the metabolic variation in vegetative, differentiated and undifferentiated mycelia. Our results revealed that during maturation, the metabolic levels of N2-acetyl-L-ornithine, 3,1-OH2-Gamma-carotene, 5Z,7E-1S,3R-24,24-difluoro-24a-homo-9,10-seco-5,7,1019-cholestatrien-1,3,25-triol, stoloniferone O, PAO-18:0-12:0, PAP-16:0-14:0, PAP-16:0-16:19Z and PAP-16:0-17:29Z,12Z were suppressed in both differentiated and undifferentiated mycelia. The concentrations of PE20:111Z-14:19Z, PEP-16:0-20:45Z,8Z,11Z,13E15OHS and PS12:0-18:19Z were increased in the differentiated group, while increased levels of Ngamma-nitro-L-arginine, tenuazonic acid and 9S,10S,11R-trihydroxy-12Z,15Z-octadecadienoic acid were found in the undifferentiated group. Our results suggest that different levels of these metabolites may act as biomarkers for the developmental stages of R. solani AG-1-IA. Moreover, the mechanisms of sclerotium formation and mycelium differentiation were elucidated at the metabolic level.



Author: Wenjin Hu, Xinli Pan, Hafiz Muhammad Khalid Abbas, Fengfeng Li, Wubei Dong

Source: http://plos.srce.hr/



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