Ebola virus RNA detection on fomites in close proximity to confirmed Ebola patients; N’Zerekore, Guinea, 2015Report as inadecuate




Ebola virus RNA detection on fomites in close proximity to confirmed Ebola patients; N’Zerekore, Guinea, 2015 - Download this document for free, or read online. Document in PDF available to download.

Objective

Health care workers HCWs in contact with patients with Ebola virus disease EVD are exposed to a risk of viral contamination. Fomites contaminated with the patient’s blood or body fluids represents this risk. Our study aims to detect Ebola virus EBOV RNA within the high- and low-risk areas of an Ebola treatment unit ETU located in inland Guinea during the 2014–2015 West African Ebola epidemics. For samples from patients’ immediate vicinity, we aim to seek an association between viral RNA detectability and level of plasma viral load of patients intermediate to high, or very high.

Methods

Swabbing was performed on immediate vicinity of Ebola patients, on surfaces of an ETU, and on personal protective equipment PPE of HCWs after patient care and prior to doffing. All samples were assessed by quantitative reverse-transcribed PCR RT-qPCR.

Results

32% 22-68 of swabs from high-risk areas were tested positive for EBOV RNA, including 42% 18-43 from patients’ immediate vicinity, and 16% 4-25 from HCWs PPE. None of specimens from low-risk areas were tested positive 0-19. Swabs were much more often viral RNA positive in the vicinity of patients with a very high plasma viral load OR 6.7, 95% CI 1.7–23.4.

Conclusion

Our findings show the persistence of EBOV RNA in the environment of Ebola patients and of HCWs, in a Guinean ETU, despite strict infection prevention and control measures. This detection raises the possibility that patients’ environment could be a potential source of contamination with the virus.



Author: Romain Palich , Leonid M. Irenge , Eric Barte de Sainte Fare, Augustin Augier, Denis Malvy , Jean-Luc Gala

Source: http://plos.srce.hr/



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