Isolation and characterization of canine perivascular stem-stromal cells for bone tissue engineeringReport as inadecuate

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For over 15 years, human subcutaneous adipose tissue has been recognized as a rich source of tissue resident mesenchymal stem-stromal cells MSC. The isolation of perivascular progenitor cells from human adipose tissue by a cell sorting strategy was first published in 2008. Since this time, the interest in using pericytes and related perivascular stem-stromal cell PSC populations for tissue engineering has significantly increased. Here, we describe a set of experiments identifying, isolating and characterizing PSC from canine tissue N = 12 canine adipose tissue samples. Results showed that the same antibodies used for human PSC identification and isolation are cross-reactive with canine tissue CD45, CD146, CD34. Like their human correlate, canine PSC demonstrate characteristics of MSC including cell surface marker expression, colony forming unit-fibroblast CFU-F inclusion, and osteogenic differentiation potential. As well, canine PSC respond to osteoinductive signals in a similar fashion as do human PSC, such as the secreted differentiation factor NEL-Like Molecule-1 NELL-1. Nevertheless, important differences exist between human and canine PSC, including differences in baseline osteogenic potential. In summary, canine PSC represent a multipotent mesenchymogenic cell source for future translational efforts in tissue engineering.

Author: Aaron W. James , Xinli Zhang , Mihaela Crisan , Winters R. Hardy, Pei Liang, Carolyn A. Meyers, Sonja Lobo, Venu Lagishetty, Mart



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