Overexpression of an EaZIP gene devoid of transit peptide sequence induced leaf variegation in tobaccoReportar como inadecuado




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Leaf variegation is an ornamental trait that is not only biologically but also economically important. In our previous study, a Mg-protoporphyrin IX monomethyl ester cyclase homologue, EaZIP Epipremnum aureum leucine zipper was found to be associated with leaf variegation in Epipremnum aureum Linden and Andre G.S. Bunting. The protein product of this nuclear-encoded gene is targeted back to chloroplast involving in chlorophyll biosynthesis. Based on a web-based homology analysis, the EaZIP was found to lack a chloroplast transit peptide cTP sequence. In the present study, we tested if overexpression of the EaZIP cDNA with or without the cTP sequence could affect leaf variegation. Transgenic tobacco plants overexpressing EaZIP genes with EaZIPwcTP and without EaZIPwocTP cTP sequence were generated. Many plant lines harboring EaZIPwocTP showed variegated leaves, while none of the plant lines with EaZIPwcTP produced such a phenotype. Molecular analysis of T0 plants and selfed T1 progeny, as well as observations of tagged marker GFP green fluorescent protein did not show any other difference in patterns of gene integrity and expression. Results from this study indicate that transgenic approach for expressing EaZIPwocTP could be a novel method of generating variegated plants even through the underlying mechanisms remain to be elucidated.



Autor: Xiayu Guan, Zhijian Li, Zhiliang Zhang, Xiangying Wei, Jiahua Xie, Jianjun Chen , Qingxi Chen

Fuente: http://plos.srce.hr/



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