Expression profiling of Chrysanthemum crassum under salinity stress and the initiation of morphological changesReportar como inadecuado




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Chrysanthemum crassum is a decaploid species of Chrysanthemum with high stress tolerance that allows survival under salinity stress while maintaining a relatively ideal growth rate. We previously recorded morphological changes after salt treatment, such as the expansion of leaf cells. To explore the underlying salinity tolerance mechanisms, we used an Illumina platform and obtained three sequencing libraries from samples collected after 0 h, 12 h and 24 h of salt treatment. Following de novo assembly, 154,944 transcripts were generated, and 97,833 63.14% transcripts were annotated, including 55 Gene Ontology GO terms and 128 Kyoto Encyclopedia of Genes and Genomes KEGG pathways. The expression profile of C. crassum was globally altered after salt treatment. We selected functional genes and pathways that may contribute to salinity tolerance and identified some factors involved in the salinity tolerance strategies of C. crassum, such as signal transduction, transcription factors and plant hormone regulation, enhancement of energy metabolism, functional proteins and osmolyte synthesis, reactive oxygen species ROS scavenging, photosystem protection and recovery, and cell wall protein modifications. Forty-six genes were selected for quantitative real-time polymerase chain reaction detection, and their expression patterns were shown to be consistent with the changes in their transcript abundance determined by RNA sequencing.



Autor: Zhiyong Guan , Yitong Feng, Aiping Song, Xiaomeng Shi, Yachao Mao, Sumei Chen, Jiafu Jiang, Lian Ding, Fadi Chen

Fuente: http://plos.srce.hr/



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