Quantitative analysis of the therapeutic effect of magnolol on MPTP-induced mouse model of Parkinson’s disease using in vivo 18F-9-fluoropropyl- -dihydrotetrabenazine PET imagingReportar como inadecuado




Quantitative analysis of the therapeutic effect of magnolol on MPTP-induced mouse model of Parkinson’s disease using in vivo 18F-9-fluoropropyl- -dihydrotetrabenazine PET imaging - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

18F-9-Fluoropropyl-+-dihydrotetrabenazine 18F-FP-+-DTBZ positron emission tomography PET has been shown to detect dopaminergic neuron loss associated with Parkinson’s disease PD in human and neurotoxin-induced animal models. A polyphenol compound, magnolol, was recently proposed as having a potentially restorative effect in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine MPTP- or 6-hydroxydopamine-treated animal models. In this study, 18F-FP-+-DTBZ PET was used to determine the therapeutic efficacy of magnolol in an MPTP–PD mouse model that was prepared by giving an intraperitoneally i.p. daily dose of 25 mg-kg MPTP to male C57BL-6 mice for 5 consecutive days. Twenty-minute static 18F-FP-+-DTBZ PET scans were performed before MPTP treatment and 5 days after the termination of MPTP treatment to set up the baseline control. Half of the MPTP-treated mice then received a daily dose of magnolol 10 mg-kg dissolved in corn oil, i.p. for 6 days. 18F-FP-+-DTBZ PET imaging was performed the day after the final treatment. All 18F-FP-+-DTBZ PET images were analysed and the specific uptake ratio SUr was calculated. Ex vivo autoradiography ARG and corresponding immunohistochemistry IHC studies were conducted to confirm the distribution of dopaminergic terminals in the striatum. The striatal SUr ratios of 18F-FP-+-DTBZ PET images for the Sham, the MPTP, and the MPTP + Magnolol-treated groups were 1.25 ± 0.05, 0.75 ± 0.06, and 1.00 ± 0.11, respectively n = 4 for each group. The ex vivo 18F-FP-+-DTBZ ARG and IHC results correlated favourably with the PET imaging results. 18F-FP-+-DTBZ PET imaging suggested that magnolol post-treatment may reverse the neuronal damage in the MPTP-lesioned PD mice. In vivo imaging of the striatal vesicular monoamine transporter type 2 VMAT2 distribution using 18F-FP-+-DTBZ animal PET is a useful method to evaluate the efficacy of therapeutic drugs i.e., magnolol, for the management of PD.



Autor: Chi-Chang Weng , Zi-An Chen , Ko-Ting Chao, Ting-Wei Ee, Kun-Ju Lin, Ming-Huan Chan, Ing-Tsung Hsiao, Tzu-Chen Yen, Mei-Ping Kung

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados