Ascorbic acid metabolism during sweet cherry Prunus avium fruit developmentReportar como inadecuado




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To elucidate metabolism of ascorbic acid AsA in sweet cherry fruit Prunus avium ‘Hongdeng’, we quantified AsA concentration, cloned sequences involved in AsA metabolism and investigated their mRNA expression levels, and determined the activity levels of selected enzymes during fruit development and maturation. We found that AsA concentration was highest at the petal-fall period 0 days after anthesis and decreased progressively during ripening, but with a slight increase at maturity. AsA did nevertheless continue to accumulate over time because of the increase in fruit fresh weight. Full-length cDNAs of 10 genes involved in the L-galactose pathway of AsA biosynthesis and 10 involved in recycling were obtained. Gene expression patterns of GDP-L-galactose phosphorylase GGP2, L-galactono-1, 4-lactone dehydrogenase GalLDH, ascorbate peroxidase APX3, ascorbate oxidase AO2, glutathione reductase GR1, and dehydroascorbate reductase DHAR1 were in accordance with the AsA concentration pattern during fruit development, indicating that genes involved in ascorbic acid biosynthesis, degradation, and recycling worked in concert to regulate ascorbic acid accumulation in sweet cherry fruit.



Autor: Dong Liang , Tingting Zhu , Zhiyou Ni, Lijin Lin, Yi Tang, Zhihui Wang, Xun Wang, Jin Wang, Xiulan Lv , Hui Xia

Fuente: http://plos.srce.hr/



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