Evaluation of a thermostable Newcastle disease virus strain TS09-C as an in-ovo vaccine for chickensReportar como inadecuado




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In-ovo vaccination is an attractive immunization approach for poultry industry. However, most of the Newcastle disease virus NDV vaccine strains used after hatch are unsafe, as in-ovo vaccines, due to their high pathogenicity for chicken embryos. In this study, we evaluated the safety and immunogenicity of a thermostable NDV strain TS09-C, derived from V4 strain, as in-ovo vaccine. Chickens in-ovo vaccinated with the parental V4 strain displayed greatly reduced hatchability and severe histopathological lesions in both trachea and intestine tissues, while the hatchability was not affected by in-ovo vaccination withTS09-C strain. The safe dose that infected all chicken embryos without obviously histopathological lesions was 103.0 EID50 per bird. In-ovo vaccination of chickens with TS09-C virus conferred complete protection against virulent NDV challenge. Results suggest that the thermostable NDV strain TS09-C is a safe and immunogenic in-ovo vaccine candidate that can be delivered quickly and uniformly, and induce earlier immune response.



Autor: Guoyuan Wen , Lintao Li , Qingzhong Yu, Hongling Wang, Qingping Luo, Tengfei Zhang, Rongrong Zhang, Wanpo Zhang , Huabin Shao

Fuente: http://plos.srce.hr/



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