A Re-Annotation of the Saccharomyces cerevisiae GenomeReport as inadecuate

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Comparative and Functional Genomics - Volume 2 2001, Issue 3, Pages 143-154

Research Article The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK

Received 14 March 2001; Accepted 19 April 2001

Copyright © 2001 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Discrepancies in gene and orphan number indicated by previous analyses suggest thatS. cerevisiae would benefit from a consistent re-annotation. In this analysis three new genesare identified and 46 alterations to gene coordinates are described. 370 ORFs are definedas totally spurious ORFs which should be disregarded. At least a further 193 genes couldbe described as very hypothetical, based on a number of criteria.It was found that disparate genes with sequence overlaps over ten amino acids especiallyat the N-terminus are rare in both S. cerevisiae and Sz. pombe. A new S. cerevisiae genenumber estimate with an upper limit of 5804 is proposed, but after the removal of veryhypothetical genes and pseudogenes this is reduced to 5570. Although this is likely to becloser to the true upper limit, it is still predicted to be an overestimate of gene number. Acomplete list of revised gene coordinates is available from the Sanger Centre S. cerevisiaereannotation: ftp:-ftp-pub-yeast-SCreannotation.

Author: V. Wood, K. M. Rutherford, A Ivens, M-A Rajandream, and B. Barrell

Source: https://www.hindawi.com/


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