Demineralized bone matrix used for direct pulp capping in ratsReport as inadecuate

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To evaluate the wound healing process following direct pulp capping with demineralized bone matrix DBM and calcium hydroxide CaOH2.


Fifty 8-weeks-old SPF Wistar male rats were divided into two groups: one was the DBM treated group, and the other was the CaOH2 treated group. Pulpotomy was performed on the maxillary first molar of one side of each rat, and the another side was left as the blank control. Rats were sacrificed after each observation period 1, 3, 7, 14 and 28 days and specimen slices were made. Hematoxylin-Eosin HE staining was used for observing the changes of pulp tissue, and immunohistochemical staining was used for observing the expression of reparative dentinogenesis-related factors runt transcription factor 2 Runx2, type I collagen COL I, osteocalcin OCN and dentin sialoprotein DSP.


Inflammatory cell infiltration ICI and pulp tissue disorganization PTD could be observed in both the DBM and CaOH2 groups at all observation periods. The DBM group showed slighter ICI on 1 and 28 days and milder PTD on 28 days, with a significant difference P<0.05. Reparative dentin formation RDF could initially be observed on 14 days postoperatively, and the DBM group showed more regular and thinner RDF with significant differences on 14 and 28 days compared with the CaOH2 group P<0.05. In both groups, the expression of Runx2, COL I, DSP and OCN were positive. Generally, the expression of these four factors in the DBM group was stronger than the CaOH2 group on the same observation periods.


DBM had the ability of inducing odontoblast differentiation and promoting dentinogenesis. DBM could initiate physiologic wound healing in pulp and had the ability to promote reparative dentin formation. Consequently, DBM may be an acceptable alternative for direct pulp capping.

Author: Qian Liu , Yanhong Ma , Junlan Wang, Xuefang Zhu, Yanjing Yang, Yufeng Mei



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