The distinct features of microbial ‘dysbiosis’ of Crohn’s disease do not occur to the same extent in their unaffected, genetically-linked kindredReport as inadecuate




The distinct features of microbial ‘dysbiosis’ of Crohn’s disease do not occur to the same extent in their unaffected, genetically-linked kindred - Download this document for free, or read online. Document in PDF available to download.

Background-Aims

Studying the gut microbiota in unaffected relatives of people with Crohn’s disease CD may advance our understanding of the role of bacteria in disease aetiology.

Methods

Faecal microbiota composition 16S rRNA gene sequencing, genetic functional capacity shotgun metagenomics and faecal short chain fatty acids SCFA were compared in unaffected adult relatives of CD children CDR, n = 17 and adult healthy controls, unrelated to CD patients HUC, n = 14. The microbiota characteristics of 19 CD children were used as a benchmark of CD ‘dysbiosis’.

Results

The CDR microbiota was less diverse p = 0.044 than that of the HUC group. Local contribution of β-diversity analysis showed no difference in community structure between the CDR and HUC groups. Twenty one of 1,243 1.8% operational taxonomic units discriminated CDR from HUC. The metagenomic functional capacity p = 0.207 and SCFA concentration or pattern were similar between CDR and HUC p>0.05 for all SCFA. None of the KEGG metabolic pathways were different between these two groups. Both of these groups HUC and CDR had a higher microbiota α-diversity CDR, p = 0.026 and HUC, p<0.001 with a community structure β-diversity distinct from that of children with CD.

Conclusions

While some alterations were observed, a distinct microbial ‘dysbiosis’, characteristic of CD patients, was not observed in their unaffected, genetically linked kindred.



Author: Umer Zeeshan Ijaz, Christopher Quince, Laura Hanske, Nick Loman, Szymon T. Calus, Martin Bertz, Christine A. Edwards, Daniel R. G

Source: http://plos.srce.hr/



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