Influence of HLA class I, HLA class II and KIRs on vertical transmission and chronicity of hepatitis C virus in childrenReport as inadecuate




Influence of HLA class I, HLA class II and KIRs on vertical transmission and chronicity of hepatitis C virus in children - Download this document for free, or read online. Document in PDF available to download.

Background and aim

There is evidence that maternal viral load of HCV during delivery influences the risk for Mother-to-child transmission MTCT, but this does not explain all cases. We study the role of the immunogenetic profile HLA, KIRs and KIR-ligand binding of mothers and children in HCV-MTCT and in chronicity in the children.

Methodology

79 HCV-RNA + mothers and their 98 children were included. 24 children were infected, becoming chronic in 8 cases and clearing in 16. HLA-class-I and II and KIRs were determined by Luminex.

Results

MTCT study: The presence of HLA-C1-ligand in mothers and-or their children reduces the risk of transmission mothers: Pc = 0.011, children: P = 0.033, whereas the presence of HLA-C2C2-ligand in mothers increases it Pc = 0.011. In children KIR2DL3-HLA-C1 is a protector factor Pc = 0.011. Chronicity in children study: Maternal DQA1*01 allele Pc = 0.027, KIR2DS1 Pc = 0.011 or KIR3DS1 Pc = 0.011 favours chronicity in the child. The presence of the DQB1*03 allele Pc = 0.027 and KIR2DS3 P = 0.056 in the child and homozygosity for KIR3DL1-3DL1 Pc = 0.011 and for the HLA-Bw4-Bw4 ligand P = 0.027 is associated with viral clearance, whereas the presence of HLA-Bw6 ligand P = 0.027, the binding of KIR3DS1-HLA-Bw4 P = 0.037 and heterozygosity for KIR3DL1-3DS1 Pc = 0.011 favour viral chronicity. Mother-child allele matching: In the joint HLA analysis, matching was greater between mothers and children with chronic infection vs those who had cleared the virus 67%±4.1 vs 57%±1.2, P = 0.003.

Conclusions

The HLA-C1 ligand in the mother is related to MTCT, while several genetic factors of the mother or child are involved in the chronification or clearance of infection in the child. Matching allelic data is considered to be an indicator of HCV chronicity in the child and can be used as a potential prognostic test. This implies that NK cells may play a previously undocumented role in protecting against MTCT and that both NK cell immunity and adaptive T-cell responses may influence viral clearance in infected children.



Author: A. Ruiz-Extremera , E. J. Pavón-Castillero , M. Florido, P. Muñoz de Rueda, J. A. Muñoz-Gámez, J. Casado, A. Carazo, R. Quile

Source: http://plos.srce.hr/



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