A multifunctional bioactive material that stimulates osteogenesis and promotes the vascularization bone marrow stem cells and their resistance to bacterial infectionReport as inadecuate




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The main limitation of tissue engineering lies in the inability to stimulate osteogenesis, angiogenesis of stem cells and broad-spectrum antimicrobial activity. However, the development of multifunctional bioactive materials with these capabilities remains a great challenge. In this study, we prepared mesoporous silica nanoparticles encapsulated with silver nanocrystals AG-MSN with uniform sphere size and mesopores. Platelet-derived growth factor BB PDGF-BB was effectively loaded in the AG-MSN mesopores P-AG-MSN. The silicon ions Si released by P-AG-MSN stimulate osteogenic differentiation of bone marrow stromal cells BMSC by activating the alkaline phosphatase ALP activity of bone-related genes and increasing protein OCN, RUNX2 and OPN expression. Ag+ ions could be slowly released from the interior of the shell, highlighting their durable antibacterial activity. The sustained release of PDGF-BB from P-AG-MSN stimulated the angiogenic differentiation of BMSC, as indicated by the enhanced secretion of vascular endothelial growth factor VEGF, HIF-1α, HGF and ANG-1 and protein expression. Our results show that P-AG-MSN can clearly promote BMSC osteostimulation and vascularization. This research serves as a preliminary study of the utilization of this multifunctional mixture to fabricate a new active biological scaffold that integrates BMSC osteostimulation, vascularization and bactericidal effects by 3D printing technology.



Author: Chuang Ma , Qin Wei , Bo Cao, Xinchun Cheng, Juling Tian, Hongwei Pu, Aihemaitijiang Yusufu , Li Cao

Source: http://plos.srce.hr/



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