Phytochemistry and Preliminary Assessment of the Antibacterial Activity of Chloroform Extract of Amburana cearensis Allemão A.C. Sm. against Klebsiella pneumoniae Carbapenemase-Producing StrainsReport as inadecuate




Phytochemistry and Preliminary Assessment of the Antibacterial Activity of Chloroform Extract of Amburana cearensis Allemão A.C. Sm. against Klebsiella pneumoniae Carbapenemase-Producing Strains - Download this document for free, or read online. Document in PDF available to download.

Evidence-Based Complementary and Alternative Medicine - Volume 2014 2014, Article ID 786586, 7 pages -

Research Article

Departamento de Biologia, Universidade Federal Rural de Pernambuco, R. Dom Manoel de Medeiros s-n, Campus Dois Irmãos, 52171-900 Recife, PE, Brazil

Departamento de Ciências Moleculares, Universidade Federal Rural de Pernambuco, R. Dom Manoel de Medeiros s-n, Campus Dois Irmãos, 52171-900 Recife, PE, Brazil

Departamento de Biofísica, Universidade Federal Rural de Pernambuco, R. Dom Manoel de Medeiros s-n, Campus Dois Irmãos, 52171-900 Recife, PE, Brazil

Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, R. Dom Manoel de Medeiros s-n, Campus Dois Irmãos, 52171-900 Recife, PE, Brazil

Laboratório de Microbiologia e Imunologia, Departamento de Biologia, Universidade Federal Rural de Pernambuco, R. Dom Manoel de Medeiros s-n, Campus Dois Irmãos, 52171-900 Recife, PE, Brazil

Received 24 November 2013; Revised 7 January 2014; Accepted 10 January 2014; Published 17 March 2014

Academic Editor: Ulysses Paulino Albuquerque

Copyright © 2014 Mirivaldo Barros Sá et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The chloroform extract of the stem bark of Amburana cearensis was chemically characterized and tested for antibacterial activity.The extract was analyzed by gas chromatography and mass spectrometry. The main compounds identified were 4-methoxy-3-methylphenol 76.7%, triciclene 3.9%, α-pinene 1.0%, β-pinene 2.2%, and 4-hydroxybenzoic acid 3.1%. Preliminary antibacterial tests were carried out against species of distinct morphophysiological characteristics: Escherichia coli, Salmonella enterica Serotype Typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus. The minimum inhibitory concentration MIC was determinate in 96-well microplates for the chloroform extract and an analogue of themain compound identified, which was purchased commercially.We have shown that plant’s extract was only inhibitory but not bactericidal at the maximum concentration of 6900 μg-mL against Pseudomonas aeruginosa and Bacillus cereus. Conversely, the analogue 2-methoxy-4-methylphenol produced MICs ranging from215 to 431 μg-mL against all bacterial species.New antibacterial assays conducted with such chemical compound against Klebsiella pneumoniae carbapenemase-producing strains have shown similarMICresults and minimumbactericidal concentration MBC of 431 μg-mL.We conclude that A. cearensis is a good source of methoxy-methylphenol compounds,which could be screened for antibacterial activity againstmultiresistant bacteria fromdifferent species





Author: Mirivaldo Barros Sá, Maria Taciana Ralph, Danielle Cristina Oliveira Nascimento, Clécio Souza Ramos, Isvânia Maria Serafin

Source: https://www.hindawi.com/



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