SlMAPK3 enhances tolerance to tomato yellow leaf curl virus TYLCV by regulating salicylic acid and jasmonic acid signaling in tomato Solanum lycopersicumReport as inadecuate




SlMAPK3 enhances tolerance to tomato yellow leaf curl virus TYLCV by regulating salicylic acid and jasmonic acid signaling in tomato Solanum lycopersicum - Download this document for free, or read online. Document in PDF available to download.

Several recent studies have reported on the role of mitogen-activated protein kinase MAPK3 in plant immune responses. However, little is known about how MAPK3 functions in tomato Solanum lycopersicum L. infected with tomato yellow leaf curl virus TYLCV. There is also uncertainty about the connection between plant MAPK3 and the salicylic acid SA and jasmonic acid JA defense-signaling pathways. The results of this study indicated that SlMAPK3 participates in the antiviral response against TYLCV. Tomato seedlings were inoculated with TYLCV to investigate the possible roles of SlMAPK1, SlMAPK2, and SlMAPK3 against this virus. Inoculation with TYLCV strongly induced the expression and the activity of all three genes. Silencing of SlMAPK1, SlMAPK2, and SlMAPK3 reduced tolerance to TYLCV, increased leaf H2O2 concentrations, and attenuated expression of defense-related genes after TYLCV infection, especially in SlMAPK3-silenced plants. Exogenous SA and methyl jasmonic acid MeJA both significantly induced SlMAPK3 expression in tomato leaves. Over-expression of SlMAPK3 increased the transcript levels of SA-JA-mediated defense-related genes PR1, PR1b-SlLapA, SlPI-I, and SlPI-II and enhanced tolerance to TYLCV. After TYLCV inoculation, the leaves of SlMAPK3 over-expressed plants compared with wild type plants showed less H2O2 accumulation and greater superoxide dismutase SOD, peroxidase POD, catalase CAT, and ascorbate peroxidase APX activity. Overall, the results suggested that SlMAPK3 participates in the antiviral response of tomato to TYLCV, and that this process may be through either the SA or JA defense-signaling pathways.



Author: Yunzhou Li, Lei Qin, Jingjing Zhao, Tayeb Muhammad, Hehe Cao, Hailiang Li, Yan Zhang , Yan Liang

Source: http://plos.srce.hr/



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