Hepatic transcriptome analysis and identification of differentially expressed genes response to dietary oxidized fish oil in loach Misgurnus anguillicaudatusReport as inadecuate




Hepatic transcriptome analysis and identification of differentially expressed genes response to dietary oxidized fish oil in loach Misgurnus anguillicaudatus - Download this document for free, or read online. Document in PDF available to download.

RNA sequencing and short-read assembly were utilized to produce a transcriptome of livers from loaches Misgurnus anguillicaudatus fed with three different diets respectively containing fresh fish oil FO group, medium oxidized fish oil MO group and high oxidized fish oil HO group. A total of 60,663 unigenes were obtained in this study, with mean length 848.74 bp. 50,814, 49,584 and 49,814 unigenes were respectively obtained from FO, MO and HO groups. There were 2,343 differentially expressed genes between FO and MO, with 855 down- and 1,488 up-regulated genes in the MO group. 2,813 genes were differentially expressed between FO and HO, including 1,256 down- and 1,552 up-regulated genes in the HO group. 2,075 differentially expressed genes were found in the comparison of MO and HO, including 1,074 up- and 1,001 down-regulated genes in the MO group. Some differentially expressed genes, such as fatty acid transport protein fatp, fatty acid binding protein fabp, apolipoprotein apo, peroxisome proliferator activated receptor-gamma ppar-γ, acetyl-CoA synthetase acs and arachidonate 5-lipoxygenase alox5, were involved in lipid metabolism, suggesting these genes in the loach were responsive to dietary oxidized fish oil. Results of transcriptome profilings here were validated using quantitative real time PCR in fourteen randomly selected unigenes. The present study provides insights into hepatic transcriptome profile of the loach, which is a valuable resource for studies of loach genomics. More importantly, this study identifies some important genes responsible for dietary oxidized fish oil, which will benefit researches of lipid metabolism in fish.



Author: Yin Zhang, Yang Li, Xiao Liang, Xiaojuan Cao, Longfei Huang, Jie Yan, Yanxing Wei, Jian Gao

Source: http://plos.srce.hr/



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