Moringa oleifera Flower Extract Suppresses the Activation of Inflammatory Mediators in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages via NF-κB PathwayReport as inadecuate




Moringa oleifera Flower Extract Suppresses the Activation of Inflammatory Mediators in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages via NF-κB Pathway - Download this document for free, or read online. Document in PDF available to download.

Mediators of Inflammation - Volume 2015 2015, Article ID 720171, 11 pages -

Research Article

Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

Received 30 June 2015; Revised 16 September 2015; Accepted 17 September 2015

Academic Editor: Barbara Romano

Copyright © 2015 Woan Sean Tan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim of Study. Moringa oleifera Lam. M. oleifera possess highest concentration of antioxidant bioactive compounds and is anticipated to be used as an alternative medicine for inflammation. In the present study, we investigated the anti-inflammatory activity of 80% hydroethanolic extract of M. oleifera flower on proinflammatory mediators and cytokines produced in lipopolysaccharide- LPS- induced RAW 264.7 macrophages. Materials and Methods. Cell cytotoxicity was conducted by 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide MTT assay. Nitric oxide NO production was quantified through Griess reaction while proinflammatory cytokines and other key inflammatory markers were assessed through enzyme-linked immunosorbent assay ELISA and immunoblotting. Results. Hydroethanolic extract of M. oleifera flower significantly suppressed the secretion and expression of NO, prostaglandin E2 PGE2, interleukin- IL- 6, IL-1β, tumor necrosis factor-alpha TNF-α, nuclear factor-kappa B NF-κB, inducible NO synthase iNOS, and cyclooxygenase-2 COX-2. However, it significantly increased the production of IL-10 and IκB-α inhibitor of κB in a concentration dependent manner 100 μg-mL and 200 μg-mL. Conclusion. These results suggest that 80% hydroethanolic extract of M. oleifera flower has anti-inflammatory action related to its inhibition of NO, PGE2, proinflammatory cytokines, and inflammatory mediator’s production in LPS-stimulated macrophages through preventing degradation of IκB-α in NF-κB signaling pathway.





Author: Woan Sean Tan, Palanisamy Arulselvan, Govindarajan Karthivashan, and Sharida Fakurazi

Source: https://www.hindawi.com/



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