Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density BarrierReport as inadecuate




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TheScientificWorldJOURNAL - Volume 2 2002, Pages 1654-1656

Peer-Reviewed Protocol School of Biomolecular Sciences, Liverpool John Moores University, UK

Received 7 March 2002; Revised 14 May 2002; Accepted 15 May 2002

Copyright © 2002 John Graham.

Abstract

The standard techniques for the isolation of human peripheral blood mononuclear cells PBMCs using commercial “lymphocyte isolation media” cannot be satisfactorily extended to experimental animals without manipulating either the density or the osmolality of the medium. PBMCs from Macaques can also be isolated from whole blood by sedimentation on to a density barrier containing approx. 10% iodixanol, polysucrose Ficoll with a density of approx 1.074 g-ml.





Author: John Graham

Source: https://www.hindawi.com/



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