Characterisation and genome sequence of the lytic Acinetobacter baumannii bacteriophage vB AbaS LokiReport as inadecuate




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Acinetobacter baumannii has emerged as an important nosocomial pathogen in healthcare and community settings. While over 100 of Acinetobacter phages have been described in the literature, relatively few have been sequenced. This work describes the characterisation and genome annotation of a new lytic Acinetobacter siphovirus, vB AbaS Loki, isolated from activated sewage sludge. Sequencing revealed that Loki encapsulates a 41,308 bp genome, encoding 51 predicted open reading frames. Loki is most closely related to Acinetobacter phage IME AB3 and more distantly related to Burkholderia phage KL1, Paracoccus phage vB PmaS IMEP1 and Pseudomonas phages vB Pae Kakheti25, vB PaeS SCH Ab26 and PA73. Loki is characterised by a narrow host range, among the 40 Acinetobacter isolates tested, productive infection was only observed for the propagating host, A. baumannii ATCC 17978. Plaque formation was found to be dependent upon the presence of Ca2+ ions and adsorption to host cells was abolished upon incubation with a mutant of ATCC 17978 encoding a premature stop codon in lpxA. The complete genome sequence of vB AbaS Loki was deposited in the European Nucleotide Archive ENA under the accession number LN890663.



Author: Dann Turner, Matthew E. Wand, Yves Briers, Rob Lavigne, J. Mark Sutton, Darren M. Reynolds

Source: http://plos.srce.hr/



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