De novo assembly of the sea trout Salmo trutta m. trutta skin transcriptome to identify putative genes involved in the immune response and epidermal mucus secretionReport as inadecuate




De novo assembly of the sea trout Salmo trutta m. trutta skin transcriptome to identify putative genes involved in the immune response and epidermal mucus secretion - Download this document for free, or read online. Document in PDF available to download.

In fish, the skin is a multifunctional organ and the first barrier against pathogens. Salmonids differ in their susceptibility to microorganisms due to varied skin morphology and gene expression patterns. The brown trout is a salmonid species with important commercial and ecological value in Europe. However, there is a lack of knowledge regarding the genes involved in the immune response and mucus secretion in the skin of this fish. Thus, we characterized the skin transcriptome of anadromous brown trout using next-generation sequencing NGS. A total of 1,348,306 filtered reads were obtained and assembled into 75,970 contigs. Of these contigs 48.57% were identified using BLAST tool searches against four public databases. KEGG pathway and Gene Ontology analyses revealed that 13.40% and 34.57% of the annotated transcripts, respectively, represent a variety of biological processes and functions. Among the identified KEGG Orthology categories, the best represented were signal transduction 23.28% and immune system 8.82%, with a variety of genes involved in immune pathways, implying the differentiation of immune responses in the trout skin. We also identified and transcriptionally characterized 8 types of mucin proteins–the main structural components of the mucosal layer. Moreover, 140 genes involved in mucin synthesis were identified, and 1,119 potential simple sequence repeats SSRs were detected in 3,134 transcripts.



Author: Magdalena Malachowicz , Roman Wenne, Artur Burzynski

Source: http://plos.srce.hr/



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