Neuroprotective Effects of Adipose-Derived Stem Cells Are Maintained for 3 Weeks against Ischemic Damage in the Rabbit Spinal CordReport as inadecuate




Neuroprotective Effects of Adipose-Derived Stem Cells Are Maintained for 3 Weeks against Ischemic Damage in the Rabbit Spinal Cord - Download this document for free, or read online. Document in PDF available to download.

BioMed Research International - Volume 2014 2014, Article ID 539051, 7 pages -

Research Article

Department of Neurosurgery, Dongtan Sacred Heart Hospital, College of Medicine, Hallym University, Hwaseong 445-170, Republic of Korea

Department of Anatomy and Cell Biology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea

Department of Veterinary Internal Medicine and Geriatrics, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea

Department of Neurology, Seoul National University Hospital, Seoul 110-744, Republic of Korea

Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea

Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea

Received 4 July 2013; Accepted 16 December 2013; Published 29 January 2014

Academic Editor: George Perry

Copyright © 2014 Seung Myung Moon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the previous study, we demonstrated that adipose-derived stem cells ASCs have neuroprotective effects against ischemic damage in the ventral horn of levels at 3 days after ischemia-reperfusion. In the present study, we expanded our observations for 3 weeks after ischemia-reperfusion to rule out the possibility of delayed neuronal death in several days after ischemia-reperfusion. Transient spinal cord ischemia was induced by a 15 min aortic artery occlusion in the subrenal region and rabbit ASCs were administered intrathecally into recipient rabbits immediately after reperfusion. Transplantation of ASCs improved the neurological motor functions of the hindlimb 3 weeks after ischemia-reperfusion. Similarly, the cresyl violet-positive neurons were significantly increased at 3 weeks after ischemia-reperfusion compared to that in the vehicle artificial cerebrospinal fluid-treated group. The transplantation of ASCs significantly reduced reactive microglia induced by ischemia at 3 weeks after ischemia-reperfusion. In addition, transplantation of ASCs maintained the brain-derived neurotrophic factor BDNF levels 3 weeks after ischemia-reperfusion. These results suggest that the neuroprotective effects of ASCs are maintained 3 weeks after ischemia-reperfusion by modulating microgliosis and BDNF levels in the spinal cord.





Author: Seung Myung Moon, Woosuk Kim, Jin Young Chung, Wooseok Im, Dae Young Yoo, Hyo Young Jung, Moo-Ho Won, Jung Hoon Choi, and

Source: https://www.hindawi.com/



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