miR-21 Reduces Hydrogen Peroxide-Induced Apoptosis in c-kit Cardiac Stem Cells In Vitro through PTEN-PI3K-Akt SignalingReport as inadecuate




miR-21 Reduces Hydrogen Peroxide-Induced Apoptosis in c-kit Cardiac Stem Cells In Vitro through PTEN-PI3K-Akt Signaling - Download this document for free, or read online. Document in PDF available to download.

Oxidative Medicine and Cellular Longevity - Volume 2016 2016, Article ID 5389181, 14 pages -

Research Article

Department of Cardiology, Affiliated Hospital of Zunyi Medical College, Zunyi 563000, China

Department of Anesthesiology, Zunyi Medical College, Zunyi 563000, China

Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical College, Zunyi 563000, China

Received 7 April 2016; Revised 6 July 2016; Accepted 19 July 2016

Academic Editor: Qingping Feng

Copyright © 2016 Wenwen Deng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The low survival rate of cardiac stem cells CSCs in the infarcted myocardium hampers cell therapy for ischemic cardiomyopathy. MicroRNA-21 miR-21 and one of its target proteins, PTEN, contribute to the survival and proliferation of many cell types, but their prosurvival effects in c-kit

CSC remain unclear. Thus, we hypothesized that miR-21 reduces hydrogen peroxide- H2O2- induced apoptosis in c-kit

CSC and estimated the contribution of PTEN-PI3K-Akt signaling to this oxidative circumstance. miR-21 mimics efficiently reduced H2O2-induced apoptosis in c-kit

CSC, as evidenced by the downregulation of the proapoptosis proteins caspase-3 and Bax and upregulation of the antiapoptotic Bcl-2. In addition, the gain of function of miR-21 in c-kit

CSC downregulated the protein level of PTEN although its mRNA level changed slightly; in the meantime, miR-21 overexpression also increased phospho-Akt p-Akt. The antiapoptotic effects of miR-21 were comparable with Phen bpV, the selective inhibitor of PTEN, while miR-21 inhibitor or PI3K’s inhibitor LY294002 efficiently attenuated the antiapoptotic effect of miR-21. Taken together, these results indicate that the anti-H2O2-induced apoptosis effect of miR-21 in c-kit

CSC is contributed by PTEN-PI3K-Akt signaling. miR-21 could be a potential molecule to facilitate the c-kit

CSC therapy in ischemic myocardium.





Author: Wenwen Deng, Yan Wang, Xianping Long, Ranzun Zhao, Zhenglong Wang, Zhijiang Liu, Song Cao, and Bei Shi

Source: https://www.hindawi.com/



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