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BioMed Research International - Volume 2015 2015, Article ID 538786, 18 pages -

Research Article

Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland

Department of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany

Flow Cytometry Facility, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland

German Aerospace Center, Space Agency, Königswinterer Straße 522-524, 53227 Bonn, Germany

Integrated Functional Genomics IFG, University of Muenster, Roentgenstraß 21, 48149 Muenster, Germany

KEK GmbH, Kemberger Straße 5, 06905 Bad Schmiedeberg, Germany

University of Applied Science Jena, Carl-Zeiss-Promenade 2, 07745 Jena, Germany

Zurich Center for Integrative Human Physiology ZIHP, University of Zurich, Winterthurerstraß 190, 8057 Zurich, Switzerland

Study Group “Magdeburger Arbeitsgemeinschaft für Forschung unter Raumfahrt- und Schwerelosigkeitsbedingungen” MARS, Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany

Received 14 May 2014; Revised 22 September 2014; Accepted 9 October 2014

Academic Editor: Jack J. W. A. Van Loon

Copyright © 2015 Katrin Paulsen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cells of the immune system are highly sensitive to altered gravity, and the monocyte as well as the macrophage function is proven to be impaired under microgravity conditions. In our study, we investigated the surface expression of ICAM-1 protein and expression of ICAM-1 mRNA in cells of the monocyte-macrophage system in microgravity during clinostat, parabolic flight, sounding rocket, and orbital experiments. In murine BV-2 microglial cells, we detected a downregulation of ICAM-1 expression in clinorotation experiments and a rapid and reversible downregulation in the microgravity phase of parabolic flight experiments. In contrast, ICAM-1 expression increased in macrophage-like differentiated human U937 cells during the microgravity phase of parabolic flights and in long-term microgravity provided by a 2D clinostat or during the orbital SIMBOX-Shenzhou-8 mission. In nondifferentiated U937 cells, no effect of microgravity on ICAM-1 expression could be observed during parabolic flight experiments. We conclude that disturbed immune function in microgravity could be a consequence of ICAM-1 modulation in the monocyte-macrophage system, which in turn could have a strong impact on the interaction with T lymphocytes and cell migration. Thus, ICAM-1 can be considered as a rapid-reacting and sustained gravity-regulated molecule in mammalian cells.





Autor: Katrin Paulsen, Svantje Tauber, Claudia Dumrese, Gesine Bradacs, Dana M. Simmet, Nadine Gölz, Swantje Hauschild, Christian

Fuente: https://www.hindawi.com/



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