Selection and evaluation of new reference genes for RT-qPCR analysis in Epinephelus akaara based on transcriptome dataReportar como inadecuado




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Groupers are an economically important fish species in world fishery markets. Because many studies using RT-qPCR have addressed gene expression in groupers, appropriate reference genes are required to obtain reliable and accurate results. In this study, the most suitable reference genes were identified from eleven candidate genes of one of the most valuable species, Epinephelus akaara, in a range of different experimental conditions. Using the software packages geNorm, NormFinder, BestKeeper and refFinder, three traditionally used reference genes, β-actin β-ACT, glyceraldehyde-3-phosphate dehydrogenase GAPDH, and beta-2-microglobulin B2M, were identified as not suitable for E. akaara gene expression studies, whereas two newly identified reference genes, conserved oligomeric Golgi complex subunit 5 Cog5 and brefeldin a-inhibited guanine nucleotide-exchange protein 1 ARFGEF1, could be universally applied under all the tested conditions. These data provide the foundation for more precise results in RT-qPCR studies of gene expression in E. akaara and other Epinephelus species.



Autor: Huan Wang, Xiang Zhang, Qiaohong Liu, Xiaochun Liu, Shaoxiong Ding

Fuente: http://plos.srce.hr/



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