Development of Combining of Human Bronchial Mucosa Models with XposeALI® for Exposure of Air Pollution NanoparticlesReportar como inadecuado




Development of Combining of Human Bronchial Mucosa Models with XposeALI® for Exposure of Air Pollution Nanoparticles - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

Exposure to agents via inhalation is of great concerns both in workplace environment and in the daily contact with particles in the ambient air. Reliable human airway exposure systems will most likely replace animal experiment in future toxicity assessment studies of inhaled agents.

Methods

In this study, we successfully established a combination of an exposure system XposeALI with 3D models mimicking both healthy and chronic bronchitis-like mucosa by co-culturing human primary bronchial epithelial cells PBEC and fibroblast at air-liquid interface ALI. Light-, confocal microscopy, scanning- and transmission electron microscopy, transepithelial electrical resistance TEER measurement and RT-PCR were performed to identify how the PBEC differentiated under ALI culture condition. Both models were exposed to palladium Pd nanoparticles which sized 6–10 nm, analogous to those released from modern car catalysts, at three different concentrations utilizing the XposeALI module of the PreciseInhale® exposure system.

Results

Exposing the 3D models to Pd nanoparticles induced increased secretion of IL-8, yet the chronic bronchitis-like model released significantly more IL-8 than the normal model. The levels of IL-8 in basal medium BM and apical lavage medium AM were in the same ranges, but the secretion of MMP-9 was significantly higher in the AM compared to the BM.

Conclusion

This combination of relevant human bronchial mucosa models and sophisticated exposure system can mimic in vivo conditions and serve as a useful alternative animal testing tool when studying adverse effects in humans exposed to aerosols, air pollutants or particles in an occupational setting.



Autor: Jie Ji , Anna Hedelin, Maria Malmlöf, Vadim Kessler, Gulaim Seisenbaeva, Per Gerde, Lena Palmberg

Fuente: http://plos.srce.hr/



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