Characterization of the Drug Resistance Profiles of Patients Infected with CRF07 BC Using Phenotypic Assay and Ultra-Deep PyrosequencingReportar como inadecuado




Characterization of the Drug Resistance Profiles of Patients Infected with CRF07 BC Using Phenotypic Assay and Ultra-Deep Pyrosequencing - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

The usefulness of ultra-deep pyrosequencing UDPS for the diagnosis of HIV-1 drug resistance DR remains to be determined. Previously, we reported an explosive outbreak of HIV-1 circulating recombinant form CRF 07 BC among injection drug users IDUs in Taiwan in 2004. The goal of this study was to characterize the DR of CRF07 BC strains using different assays including UDPS. Seven CRF07 BC isolates including 4 from early epidemic collected in 2004–2005 and 3 from late epidemic collected in 2008 were obtained from treatment-naïve patient’s peripheral blood mononuclear cells. Viral RNA was extracted directly from patient’s plasma or from cultural supernatant and the pol sequences were determined using RT-PCR sequencing or UDPS. For comparison, phenotypic drug susceptibility assay using MAGIC-5 cells in-house phenotypic assay and Antivirogram were performed. In-house phenotypic assay showed that all the early epidemic and none of the late epidemic CRF07 BC isolates were resistant to most protease inhibitors PIs 4.4–47.3 fold. Neither genotypic assay nor Antivirogram detected any DR mutations. UDPS showed that early epidemic isolates contained 0.01–0.08% of PI DR major mutations. Furthermore, the combinations of major and accessory PI DR mutations significantly correlated with the phenotypic DR. The in-house phenotypic assay is superior to other conventional phenotypic assays in the detection of DR variants with a frequency as low as 0.01%.



Autor: Szu-Wei Huang, Wei-You Li, Wen-Hung Wang, Yu-Ting Lin, Chih-Hung Chou, Marcelo Chen, Hsien-Da Huang, Yen-Hsu Chen, Po-Liang Lu, S

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados