MicroRNAs, miR-23a-3p and miR-151-3p, Are Regulated in Dentate Gyrus Neuropil following Induction of Long-Term Potentiation In VivoReportar como inadecuado




MicroRNAs, miR-23a-3p and miR-151-3p, Are Regulated in Dentate Gyrus Neuropil following Induction of Long-Term Potentiation In Vivo - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Translation of synaptic mRNA contributes to alterations in the proteome necessary to consolidate long-term potentiation LTP, a model of memory processes. Yet, how this process is controlled is not fully resolved. MicroRNAs are non-coding RNAs that negatively regulate gene expression by suppressing translation or promoting mRNA degradation. As specific microRNAs are synaptically located, we hypothesized that they are ideally suited to couple synaptic activation, translational regulation, and LTP persistence. The aim of this study was to identify LTP-regulated microRNAs at or near synapses. Accordingly, LTP was induced unilaterally at perforant path-dentate gyrus synapses in awake adult Sprague-Dawley rats. Five hours later, dentate gyrus middle molecular layer neuropil, containing potentiated synapses, was laser-microdissected. MicroRNA expression profiling, using TaqMan Low Density MicroRNA Microarrays n = 4, identified eight regulated microRNAs. Subsequent individual TaqMan assays confirmed upregulation of miR-23a-3p 1.30 ± 0.10; p = 0.015 and miR-151-3p 1.17 ± 0.19; p = 0.045 in a second cohort n = 7. Interestingly, bioinformatic analysis indicated that miR-151-3p and miR-23a-3p regulate synaptic reorganisation and transcription, respectively. In summary, we have demonstrated for the first time that microRNAs are regulated in isolated neuropil following LTP induction in vivo, supporting the hypothesis that synaptic, LTP-responsive microRNAs contribute to LTP persistence via regulation of the synaptic proteome.



Autor: Brigid Ryan, Barbara J. Logan, Wickliffe C. Abraham, Joanna M. Williams

Fuente: http://plos.srce.hr/



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