Modulation of Tamoxifen Cytotoxicity by Caffeic Acid Phenethyl Ester in MCF-7 Breast Cancer CellsReportar como inadecuado

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Oxidative Medicine and Cellular LongevityVolume 2016 2016, Article ID 3017108, 13 pages

Research Article

Department of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt

Department of Cancer Biology, National Cancer Institute, Cairo University, Cairo 11796, Egypt

Received 3 September 2015; Accepted 27 October 2015

Academic Editor: Mohamed A. Dkhil

Copyright © 2016 Tarek K. Motawi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Although Tamoxifen TAM is one of the most widely used drugs in managing breast cancer, many women still relapse after long-term therapy. Caffeic acid phenethyl ester CAPE is a polyphenolic compound present in many medicinal plants and in propolis. The present study examined the effect of CAPE on TAM cytotoxicity in MCF-7 cells. MCF-7 cells were treated with different concentrations of TAM and-or CAPE for 48 h. This novel combination exerted synergistic cytotoxic effects against MCF-7 cells via induction of apoptotic machinery with activation of caspases and DNA fragmentation, along with downregulation of Bcl-2 and Beclin 1 expression levels. However, the mammalian microtubule-associated protein light chain LC 3-II level was unchanged. Vascular endothelial growth factor level was also decreased, whereas levels of glutathione and nitric oxide were increased. In conclusion, CAPE augmented TAM cytotoxicity via multiple mechanisms, providing a novel therapeutic approach for breast cancer treatment that can overcome resistance and lower toxicity. This effect provides a rationale for further investigation of this combination.

Autor: Tarek K. Motawi, Samy A. Abdelazim, Hebatallah A. Darwish, Eman M. Elbaz, and Samia A. Shouman



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