Sansevieria roxburghiana Schult. and Schult. F. Family: Asparagaceae Attenuates Type 2 Diabetes and Its Associated CardiomyopathyReportar como inadecuado




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Background

Sansevieria roxburghiana Schult. and Schult. F. Family: Asparagaceae rhizome has been claimed to possess antidiabetic activity in the ethno-medicinal literature in India. Therefore, present experiments were carried out to explore the protective role of edible aqueous extract of S. roxburghiana rhizome SR against experimentally induced type 2 diabetes mellitus T2DM and its associated cardiomyopathy in Wistar rats.

Methods

SR was chemically characterized by GC-MS analysis. Antidiabetic activity of SR 50 and 100 mg-kg, orally was measured in high fat diets ad libitum + low-single dose of streptozotocin 35 mg-kg, intraperitoneal induced type 2 diabetic T2D rat. Fasting blood glucose level was measured at specific intermissions. Serum biochemical and inflammatory markers were estimated after sacrificing the animals. Besides, myocardial redox status, expressions of signal proteins NF-κB and PKCs, histological and ultrastructural studies of heart were performed in the controls and SR treated T2D rats.

Results

Phytochemical screening of the crude extract revealed the presence of phenolic compounds, sugar alcohols, sterols, amino acids, saturated fatty acids within SR. T2D rats exhibited significantly p < 0.01 higher fasting blood glucose level with respect to control. Alteration in serum lipid profile p < 0.01 and increased levels of lactate dehydrogenase p < 0.01 and creatine kinase p < 0.01 in the sera revealed the occurrence of hyperlipidemia and cell destruction in T2D rats. T2DM caused significant p < 0.05–0.01 alteration in the biochemical markers in the sera. T2DM altered the redox status p < 0.05–0.01, decreased p < 0.01 the intracellular NAD and ATP concentrations in the myocardial tissues of experimental rats. While investigating the molecular mechanism, activation PKC isoforms was observed in the selected tissues. T2D rats also exhibited an up-regulation in nuclear NF-κB p65 in the cardiac tissues. So, oral administration of SR 50 and 500 mg-kg could reduce hyperglycemia, hyperlipidemia, membrane disintegration, oxidative stress, vascular inflammation and prevented the activation of oxidative stress induced signaling cascades leading to cell death. Histological and ultra-structural studies of cardiac tissues supported the protective characteristics of SR.

Conclusions

From the present findings it can be concluded that, SR could offer protection against T2DM and its associated cardio-toxicity via multiple mechanisms viz. hypoglycemic, antioxidant and anti-inflammatory actions.



Autor: Niloy Bhattacharjee, Ritu Khanra, Tarun K. Dua, Susmita Das, Bratati De, M. Zia-Ul-Haq, Vincenzo De Feo , Saikat Dewanjee

Fuente: http://plos.srce.hr/



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