Lipopolysaccharides-Induced Suppression of Innate-Like B Cell Apoptosis Is Enhanced by CpG Oligodeoxynucleotide and Requires Toll-Like Receptors 2 and 4Reportar como inadecuado




Lipopolysaccharides-Induced Suppression of Innate-Like B Cell Apoptosis Is Enhanced by CpG Oligodeoxynucleotide and Requires Toll-Like Receptors 2 and 4 - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Innate-like B lymphocytes play an important role in innate immunity in periodontal disease through Toll-like receptor TLR signaling. However, it is unknown how innate-like B cell apoptosis is affected by the periodontal infection-associated innate signals. This study is to determine the effects of two major TLR ligands, lipopolysaccharide LPS and CpG-oligodeoxynucleotides CpG-ODN, on innate-like B cell apoptosis. Spleen B cells were isolated from wild type WT, TLR2 knockout KO and TLR4 KO mice and cultured with E. coli LPS alone, P. gingivalis LPS alone, or combined with CpG-ODN for 2 days. B cell apoptosis and expressions of specific apoptosis-related genes were analyzed by flow cytometry and real-time PCR respectively. P. gingivalis LPS, but not E. coli LPS, reduced the percentage of AnnexinV+-7-AAD- cells within IgMhighCD23lowCD43-CD93- marginal zone MZ B cell sub-population and IgMhighCD23lowCD43+CD93+ innate response activator IRA B cell sub-population in WT but not TLR2KO or TLR4KO mice. CpG-ODN combined with P. gingivalis LPS further reduced the percentage of AnnexinV+-7-AAD- cells within MZ B cells and IRA B cells in WT but not TLR2 KO or TLR4 KO mice. Pro-apoptotic CASP4, CASP9 and Dapk1 were significantly down-regulated in P. gingivalis LPS- and CpG-ODN-treated B cells from WT but not TLR2 KO or TLR4 KO mice. Anti-apoptotic IL-10 was significantly up-regulated in P. gingivalis LPS- and CpG-ODN-treated B cells from WT and TLR2 KO but not TLR4 KO mice. These results suggested that both TLR2 and TLR4 signaling are required for P. gingivalis LPS-induced, CpG-ODN-enhanced suppression of innate-like B cell apoptosis.



Autor: Xiaoqian Yu , Yuhua Wang , Jiang Lin, Yang Hu, Toshihisa Kawai, Martin A. Taubman, Xiaozhe Han

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados