Development and Application of a Microfluidics-Based Panel in the Basal-Luminal Transcriptional Characterization of Archival Bladder CancersReportar como inadecuado




Development and Application of a Microfluidics-Based Panel in the Basal-Luminal Transcriptional Characterization of Archival Bladder Cancers - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

In the age of personalized medicine stratifying tumors into molecularly defined subtypes associated with distinctive clinical behaviors and predictable responses to therapies holds tremendous value. Towards this end, we developed a custom microfluidics-based bladder cancer gene expression panel for characterization of archival clinical samples. In silico analysis indicated that the content of our panel was capable of accurately segregating bladder cancers from several public datasets into the clinically relevant basal and luminal subtypes. On a technical level, our bladder cancer panel yielded robust and reproducible results when analyzing formalin-fixed, paraffin-embedded FFPE tissues. We applied our panel in the analysis of a novel set of 204 FFPE samples that included non-muscle invasive bladder cancers NMIBCs, muscle invasive disease MIBCs, and bladder cancer metastases METs. We found NMIBCs to be mostly luminal-like, MIBCs to include both luminal- and basal-like types, and METs to be predominantly of a basal-like transcriptional profile. Mutational analysis confirmed the expected enrichment of FGFR3 mutations in luminal samples, and, consistently, FGFR3 IHC showed high protein expression levels of the receptor in these tumors. Our bladder cancer panel enables basal-luminal characterization of FFPE tissues and with further development could be used for stratification of bladder cancer samples in the clinic.



Autor: Doris Kim , YounJeong Choi , James Ireland , Oded Foreman, Rachel N. Tam, Rajesh Patel, Erica B. Schleifman, Maipelo Motlhabi, Do

Fuente: http://plos.srce.hr/



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