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aluminum, peroxidase, stress

Liu, Tianzhen

Supervisor and department: Gregory, Taylor Biological Sciences

Examining committee member and department: Randall, Weselake Agricultural, Food and Nutritional Science Allen, Good Biological Sciences

Department: Department of Biological Sciences

Specialization:

Date accepted: 2010-04-12T16:11:32Z

Graduation date: 2010-06

Degree: Master of Science

Degree level: Master's

Abstract: Class III peroxidases have been identified as secreted proteins involved in plant defense, auxin metabolism, cell wall modification, and regulation of reactive oxygen species. Transcriptome analysis of Arabidopsis has identified many class III peroxidases that respond to aluminum stress. The large number and versatile function of peroxidases make functional characterization of individual peroxidases a challenging task. However, the use of promoter::GUS reporter fusions can help to elucidate the expression pattern of individual peroxidases. To investigate the expression of PER22 and PER73 under aluminum stress in Arabidopsis, single-copy, homozygous, transgenic plants harbouring promoter::GUS reporter fusions were generated through self-pollination and southern blot analyses. Histochemical GUS staining of transgenic lines revealed trichome-specific and vascular-specific expression patterns for PER22 and PER73, respectively. The temporal and spatial expression of PER22 and PER73 suggest that they might be involved in pathogen defense and-or lignification. Further experiments will help define these peroxidase functions under aluminum stress.

Language: English

DOI: doi:10.7939-R33X4M

Rights: License granted by Tianzhen Liu tianzhen@ualberta.ca on 2010-04-10 GMT: Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.





Autor: Liu, Tianzhen

Fuente: https://era.library.ualberta.ca/


Introducción



University of Alberta Tissue-specific gene expression of two class III Arabidopsis peroxidases under aluminum stress by Tianzhen Liu A thesis submitted to the Faculty of Graduate Studies and Research in partial fulfillment of the requirements for the degree of Master of Science in Plant Biology Department of Biological Sciences ©Tianzhen Liu Spring 2010 Edmonton, Alberta Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only.
Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the authors prior written permission. Examining Committee Dr.
Gregory Taylor, Biological Sciences Dr.
Allen Good, Biological Sciences Dr.
Randall Weselake, Agricultural, Food and Nutritional Science Abstract Class III peroxidases have been identified as secreted proteins involved in plant defense, auxin metabolism, cell wall modification, and regulation of reactive oxygen species.
Transcriptome analysis of Arabidopsis has identified many class III peroxidases that respond to aluminum stress.
The large number and versatile function of peroxidases make functional characterization of individual peroxidases a challenging task.
However, the use of promoter::GUS reporter fusions can help to elucidate the expression pattern of individual peroxidases.
To investigate the expression of PER22 and PER73 under aluminum stress in Arabidopsis, single-copy, homozygous, transgenic plants harbouring promoter::GUS reporter fusions were generated through self-p...





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