Genetic Characteristics and Multiple-PCR Development for Capsular Identification of Specific Serotypes of Campylobacter jejuniReportar como inadecuado




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The polysaccharide capsule CPS of Campylobacter jejuni is a virulence factor linked to cell surface carbohydrate diversity which mainly determines the serotypes. Thirty-four CPS gene cluster structures have been published and some of them can be distinguished by multiple-PCR. Penner serotypes HS1-44c, HS2, HS4c, HS19, HS23-36c and HS41 are markers for Guillain—Barré syndrome GBS. The capsules may contribute to GBS susceptibility. Analysis of 18 CPS loci revealed high gene content diversity and a mosaic nature of the capsule loci, which are possibly due to gene gain-loss events, and demonstrated a high degree of conservation of genes within serotypes-serotype complexes. A method of multiple-PCR was developed to distinguish five specific serotypes and three GBS-related serotypes. Primers specific for each capsule type were designed on the basis of paralogs or a unique DNA region of the CPS locus. The multiple-PCR can distinguish the eight serotypes in two PCRs with sensitivity and specificity of 100% using 227 strains of known Penner type. The multiple-PCR method will help to distinguish serotypes simply and rapidly.



Autor: Hao Liang, Aiyu Zhang, Yixin Gu, Yuanhai You, Jianzhong Zhang, Maojun Zhang

Fuente: http://plos.srce.hr/



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