Nectin-2 CD112 Is Expressed on Outgrowth Endothelial Cells and Regulates Cell Proliferation and Angiogenic FunctionReportar como inadecuado




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Outgrowth endothelial cells OECs are a subpopulation of endothelial progenitor cells EPCs that have the capacity for proliferation and the ability to promote angiogenesis. In this study, we identified Nectin-2 as a surface protein of OECs through unbiased quantitative proteomics analysis. Using immunocytochemistry and flow cytometry, we confirmed that Nectin-2 is highly expressed on OECs. Nectin-2 CD112 expression was limited or lower on mononuclear cells MNCs and mature tube-forming endothelial cells ECs. Blocking Nectin-2 with a neutralizing monoclonal antibody significantly increased the trans-well migration and tube forming capacity of OECs. Similarly, Nectin-2 knockdown resulted in enhanced tube formation, cell migration and proliferation with p-Erk activation. Moreover, Nectin-2 deficiency resulted in compensatory increase of other Nectin family genes including Nectin-3 and Necl-4 which promote VEGFR signaling. These results indicate that Nectin-2 is a surface marker and an important regulator of OECs, with significant implications for the isolation of OECs and blocking Nectin-2 on OECs by an antibody for angiogenic applications.



Autor: YeonSung Son, BomNaeRin Lee, Young-Jin Choi, Seon Ae Jeon, Ju-Hyun Kim, Hoo-Keun Lee, Sang-Mo Kwon, Je-Yoel Cho

Fuente: http://plos.srce.hr/



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