SmShb, the SH2-Containing Adaptor Protein B of Schistosoma mansoni Regulates Venus Kinase Receptor Signaling PathwaysReportar como inadecuado




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Venus kinase receptors VKRs are invertebrate receptor tyrosine kinases RTKs formed by an extracellular Venus Fly Trap VFT ligand binding domain associated via a transmembrane domain with an intracellular tyrosine kinase TK domain. Schistosoma mansoni VKRs, SmVKR1 and SmVKR2, are both implicated in reproductive activities of the parasite. In this work, we show that the SH2 domain-containing protein SmShb is a partner of the phosphorylated form of SmVKR1. Expression of these proteins in Xenopus oocytes allowed us to demonstrate that the SH2 domain of SmShb interacts with the phosphotyrosine residue pY979 located in the juxtamembrane region of SmVKR1. This interaction leads to phosphorylation of SmShb on tyrosines and promotes SmVKR1 signaling towards the JNK pathway. SmShb transcripts are expressed in all parasite stages and they were found in ovary and testes of adult worms, suggesting a possible colocalization of SmShb and SmVKR1 proteins. Silencing of SmShb in adult S. mansoni resulted in an accumulation of mature sperm in testes, indicating a possible role of SmShb in gametogenesis.



Autor: Marion Morel, Mathieu Vanderstraete, Katia Cailliau, Steffen Hahnel, Christoph G. Grevelding, Colette Dissous

Fuente: http://plos.srce.hr/



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