Identification and Characterization of Cefotaxime Resistant Bacteria in Beef CattleReportar como inadecuado

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Third-generation cephalosporins are an important class of antibiotics that are widely used in treatment of serious Gram-negative bacterial infections. In this study, we report the isolation of bacteria resistant to the third-generation cephalosporin cefotaxime from cattle with no previous cefotaxime antibiotic exposure. The prevalence of cefotaxime-resistant bacteria was examined by a combination of culture based and molecular typing methods in beef cattle n = 1341 from 8 herds located in North Central Florida. The overall prevalence of cefotaxime-resistant bacteria was 15.8% 95% CI: 13.9, 17.8, varied between farms, and ranged from 5.2% to 100%. A subset of isolates n = 23 was further characterized for the cefotaxime minimum inhibitory concentration MIC and antibiotic susceptibility against 10 different antibiotics, sequencing of nine β- lactamase genes, and species identification by 16S rRNA sequencing. Most of the bacterial isolates were resistant to cefotaxime concentrations, > 64 μg-mL and showed high levels of multi-drug resistance. Full length 16S rRNA sequences ~1300 bp revealed that most of the isolates were not primary human or animal pathogens; rather were more typical of commensal, soil, or other environmental origin. Six extended spectrum β-lactamase ESBL genes identical to those in clinical human isolates were identified. Our study highlights the potential for carriage of cefotaxime resistance including -human- ESBL genes by the bacterial flora of food animals with no history of cefotaxime antibiotic exposure. A better understanding of the origin and transmission of resistance genes in these pre-harvest settings will be critical to development of strategies to prevent the spread of antimicrobial resistant microorganisms to hospitals and communities.

Autor: Raies A. Mir, Thomas A. Weppelmann, Judith A. Johnson, Douglas Archer, J. Glenn Morris Jr, KwangCheol Casey Jeong



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