Single Plant Derived Nanotechnology for Synergistic Antibacterial TherapiesReport as inadecuate

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Multiple new approaches to tackle multidrug resistant infections are urgently needed and under evaluation. One nanotechnology-based approach to delivering new relevant therapeutics involves silicon accumulator plants serving as a viable silicon source in green routes for the fabrication of the nanoscale drug delivery carrier porous silicon pSi. If the selected plant leaf components contain medicinally-active species as well, then a single substance can provide not only the nanoscale high surface area drug delivery carrier, but the drug itself. With this idea in mind, porous silicon was fabricated from joints of the silicon accumulator plant Bambuseae Tabasheer and loaded with an antibacterial extract originating from leaves of the same type of plant Bambuseae arundinacea. Preparation of porous silicon from Tabasheer includes extraction of biogenic silica from the ground plant by calcination, followed by reduction with magnesium in the presence of sodium chloride, thereby acting as a thermal moderator that helps to retain the mesoporous structure of the feedstock. The purified product was characterized by a combination of scanning electron microscopy SEM, energy dispersive X-ray analysis EDX, X-ray diffraction XRD, Raman spectroscopy, transmission electron microscopy TEM, and low temperature nitrogen gas adsorption measurements. Antimicrobial activity and minimum inhibitory concentration of a leaf extract of Bambuseae arundinacea was tested against the bacteria Escherichia Coli E. Coli and Staphylococcus aureus S. Aureus, along with the fungus Candida albicans C. Albicans. A S. aureus active ethanolic leaf extract was loaded into the above Tabasheer-derived porous silicon. Initial studies indicate sustained in vitro antibacterial activity of the extract-loaded plant derived pSi 25 wt %, TGA, as measured by disk diffusion inhibitory zone assays. Subsequent chromatographic separation of this extract revealed that the active antimicrobial species present include stigmasterol and 2,6-dimethoxy-p-benzoquinone.

Author: Jhansi R. Kalluri, Roberto Gonzalez-Rodriguez, Phil S. Hartman, Armando Loni, Leigh T. Canham, Jeffery L. Coffer



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