Activation of the Glutamic Acid-Dependent Acid Resistance System in Escherichia coli BL21DE3 Leads to Increase of the Fatty Acid Biotransformation ActivityReportar como inadecuado




Activation of the Glutamic Acid-Dependent Acid Resistance System in Escherichia coli BL21DE3 Leads to Increase of the Fatty Acid Biotransformation Activity - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

The biosynthesis of carboxylic acids including fatty acids from biomass is central in envisaged biorefinery concepts. The productivities are often, however, low due to product toxicity that hamper whole-cell biocatalyst performance. Here, we have investigated factors that influence the tolerance of Escherichia coli to medium chain carboxylic acid i.e., n-heptanoic acid-induced stress. The metabolic and genomic responses of E. coli BL21DE3 and MG1655 grown in the presence of n-heptanoic acid indicated that the GadA-B-based glutamic acid-dependent acid resistance GDAR system might be critical for cellular tolerance. The GDAR system, which is responsible for scavenging intracellular protons by catalyzing decarboxylation of glutamic acid, was inactive in E. coli BL21DE3. Activation of the GDAR system in this strain by overexpressing the rcsB and dsrA genes, of which the gene products are involved in the activation of GadE and RpoS, respectively, resulted in acid tolerance not only to HCl but also to n-heptanoic acid. Furthermore, activation of the GDAR system allowed the recombinant E. coli BL21DE3 expressing the alcohol dehydrogenase of Micrococcus luteus and the Baeyer-Villiger monooxygenase of Pseudomonas putida to reach 60% greater product concentration in the biotransformation of ricinoleic acid i.e., 12-hydroxyoctadec-9-enoic acid 1 into n-heptanoic acid 5 and 11-hydroxyundec-9-enoic acid 4. This study may contribute to engineering E. coli-based biocatalysts for the production of carboxylic acids from renewable biomass.



Autor: Ji-Min Woo, Ji-Won Kim, Ji-Won Song, Lars M. Blank, Jin-Byung Park

Fuente: http://plos.srce.hr/



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