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Evidence-Based Complementary and Alternative MedicineVolume 2013 2013, Article ID 950272, 10 pages

Research Article

Departamento de Biologia Celular e Genética, Centro de Biociências, Universidade Federal do Rio Grande do Norte, 59072-970 Natal, RN, Brazil

Laboratório de Biotecnologia de Polímeros Naturais-Biopol, Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, 59072-970 Natal, Brazil

Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, 59010-180 Natal, Brazil

Received 18 March 2013; Revised 6 July 2013; Accepted 30 July 2013

Academic Editor: José Luis Ríos

Copyright © 2013 Ana Karina Lima Nascimento et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Plukenetia volubilis Linneo, or Sacha inca, is an oleaginous plant from the Euphorbiaceae family. The aim of this work was to perform a chemical and biological analysis of different leaf extracts from P. volubilis such as aqueous extract AEL, methanol MEL, ethanol EEL, chloroform CEL, and hexane HEL. Thin layer chromatography analysis revealed the presence of phenolic compounds, steroids, and-or terpenoídes. Furthermore, the antioxidant activities were analyzed by in vitro assays and their effects on cell lineages by in vivo assays. The Total Antioxidant Capacity TCA was expressed as equivalent ascorbic acid EEA-g and it was observed that the extracts showed values ranging from 59.31 to 97.76 EAA-g. Furthermore, the DPPH assay values ranged from 62.8% to 88.3%. The cell viability assay showed that the extracts were able to reduce viability from cancer cells such as HeLa and A549 cells. The extracts MEL and HEL 250 µg-mL were able to reduce the proliferation of HeLa cells up to 54.3% and 48.5%, respectively. The flow cytometer results showed that these extracts induce cell death via the apoptosis pathway. On the other hand, the extracts HEL and AEL were able to induce cell proliferation of normal fibroblast 3T3 cells.





Autor: Ana Karina Lima Nascimento, Raniere Fagundes Melo-Silveira, Nednaldo Dantas-Santos, Júlia Morais Fernandes, Silvana Maria Zuc

Fuente: https://www.hindawi.com/



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