Titanium and Zirconium Levels Are Associated with Changes in MicroRNAs Expression: Results from a Human Cross-Sectional Study on Obese PopulationReportar como inadecuado




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Objectives

In this study on 90 individuals we aimed at evaluating the microRNAs miRNAs expression profile associated with personal levels of Titanium Ti and Zirconium Zr traced in hair samples. Ti and Zr materials are broadly used for dental implants but the biological reactions triggered by a long term presence of these materials in the oral cavity still need to be assessed. MiRNAs are mechanisms that need to be investigated as they play a fundamental role in the control of gene expression following external stimuli and contribute to a wide range of pathophysiological processes.

Methods

Using the TaqMan® Low-Density Array, we assessed the expression levels of 377 human miRNAs in peripheral blood of 90 subjects. Hair samples were analyzed for Ti and Zr content using Inductively Coupled Plasma-Mass Spectrometry. We performed multivariable regression analysis to investigate the effects of Ti and Zr exposure on miRNA expression levels. We used the Ingenuity Pathway Analysis IPA software to explore the functional role of the investigated miRNAs and the related target genes.

Results

Seven miRNAs miR-99b, miR-142-5p, miR-152, miR-193a-5p, miR-323-3p, miR-335, miR-494 resulted specifically associated with Zr levels. The functional target analysis showed that miRNAs are involved in mechanisms such as inflammation, skeletal and connective tissue disorders.

Conclusions

Our data suggest that Zr is more bioactive than Ti and show that miRNAs are relevant molecular mechanisms sensitive to Zr exposure.



Autor: Gianguido Cossellu , Valeria Motta , Laura Dioni, Laura Angelici, Luisella Vigna, Giampietro Farronato, Angela Cecilia Pesatori,

Fuente: http://plos.srce.hr/



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