Development of a TaqMan Probe-Based Insulated Isothermal Polymerase Chain Reaction iiPCR Assay for Detection of Fusarium oxysporum f. sp. cubense Race 4Reportar como inadecuado




Development of a TaqMan Probe-Based Insulated Isothermal Polymerase Chain Reaction iiPCR Assay for Detection of Fusarium oxysporum f. sp. cubense Race 4 - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

This study developed a novel and inexpensive detection method based on a TaqMan probe-based insulated isothermal polymerase chain reaction iiPCR method for the rapid detection of Panama disease caused by Fusarium oxysporum f. sp. cubense Foc race 4, which is currently among the most serious fungal vascular diseases worldwide. By using the portable POCKIT™ device with the novel primer set iiFoc-1-iiFoc-2, the Foc race 4 iiPCR assay including DNA amplification and signal monitoring could be completed within one hour. The developed Foc race 4 iiPCR assay is thus a user-friendly and efficient platform designed specifically for the detection of Foc race 4. The detection limit of this optimized Foc iiPCR system was estimated to be 1 copy of the target standard DNA as well as 1 fg of the Foc genomic DNA. This approach can serve as a rapid detection method for in planta detection of Foc race 4 in field-infected banana. It was concluded that this molecular detection procedure based on iiPCR has good potential for use as an efficient detection method.



Autor: Ying-Hong Lin , Yi-Jia Lin, Tsai-De Chang, Li-Ling Hong, Tzu-Yu Chen, Pi-Fang Linda Chang

Fuente: http://plos.srce.hr/



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