Flow Cytometric Immunophenotyping Is Sensitive for the Early Diagnosis of De Novo Aggressive Natural Killer Cell Leukemia ANKL: A Multicenter Retrospective AnalysisReportar como inadecuado




Flow Cytometric Immunophenotyping Is Sensitive for the Early Diagnosis of De Novo Aggressive Natural Killer Cell Leukemia ANKL: A Multicenter Retrospective Analysis - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Aggressive natural killer cell leukemia ANKL is a fatal hematological neoplasm characterized by a fulminating clinical course and extremely high mortality. Current diagnosis of this disease is not effective during the early stages and it is easily misdiagnosed as other NK cell disorders. We retrospectively analyzed the clinical characteristics and flow cytometric immunophenotype of 47 patients with ANKL. Patients with extranodal NK-T cell lymphoma, nasal type ENKTL and chronic lymphoproliferative disorder of NK cell CLPD-NK, who were diagnosed during the same time period were used for comparisons. Abnormal NK cells in ANKL were found to have a distinctiveCD56bright-CD16dim immunophenotype and markedly increased Ki-67 expression, whereas CD57 negativity and reduced expression of killer immunoglobulin-like receptor KIR, CD161, CD7, CD8 and perforin were exhibited compared with other NK cell proliferative disorders p<0.05. The positive rates of flow cytometry detection 97.4% was higher than those of cytomorphological 89.5%, immunohistochemical 90%, cytogenetic 56.5% and F-18 fluorodeoxyglucose positron emission tomography-computer tomography 18-FDG-PET-CT examinations 50% p<0.05. ANKL is a highly aggressive leukemia with high mortality. Flow cytometry detection is sensitive for the early and differential diagnosis of ANKL with high specificity.



Autor: Yi Li , Jia Wei , Xia Mao, Qingping Gao, Longlong Liu, Ping Cheng, Limei Liu, Xinhua Zhang, Ke Zhang, Jin Wang, Li Zhu, Jianfeng

Fuente: http://plos.srce.hr/



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