A High Malaria Prevalence Identified by PCR among Patients with Acute Undifferentiated Fever in IndiaReportar como inadecuado




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Background

Approximately one million malaria cases were reported in India in 2015, based on microscopy. This study aims to assess the malaria prevalence among hospitalised fever patients in India identified by PCR, and to evaluate the performance of routine diagnostic methods.

Methods

During June 2011-December 2012, patients admitted with acute undifferentiated fever to seven secondary level community hospitals in Assam Tezpur, Bihar Raxaul, Chhattisgarh Mungeli, Maharashtra Ratnagiri, Andhra Pradesh Anantapur and Tamil Nadu Oddanchatram and Ambur were included. The malaria prevalence was assessed by polymerase chain reaction PCR, routine microscopy, and a rapid diagnostic test RDT with PCR as a reference method.

Results

The malaria prevalence by PCR was 19% 268-1412 ranging from 6% Oddanchatram, South India to 35% Ratnagiri, West India. Among malaria positive patients P. falciparum single infection was detected in 46%, while 38% had P. vivax, 11% mixed infections with P. falciparum and P. vivax, and 5% P. malariae. Compared to PCR, microscopy had sensitivity of 29% and specificity of 98%, while the RDT had sensitivity of 24% and specificity of 99%.

Conclusions

High malaria prevalence was identified by PCR in this cohort. Routine diagnostic methods had low sensitivity compared to PCR. The results suggest that malaria is underdiagnosed in rural India. However, low parasitaemia controlled by immunity may constitute a proportion of PCR positive cases, which calls for awareness of the fact that other pathogens could be responsible for the febrile disease in submicroscopic malaria.



Autor: Christel Gill Haanshuus, Sara Chandy, Anand Manoharan , Rosario Vivek, Dilip Mathai, Deepika Xena, Ashita Singh, Nina Langeland,

Fuente: http://plos.srce.hr/



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