Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain SlicesReportar como inadecuado




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The intraneuronal chloride concentration Cl-i is paramount for determining the polarity of signaling at GABAA synapses in the central nervous system. Sectioning hippocampal brain slices increases Cl-i in the superficial layers. It is not known whether cutting trauma also increases Cl-i in the neocortex and thalamus, and whether the effects of trauma change during development. We used Cl- imaging to study the Cl-i vs. the distance from the cut surface in acute thalamocortical slices from mice at developmental ages ranging from post-natal day 5 P5 to P20. We demonstrate: 1 Cl-i is higher in the most superficial areas in both neocortical and thalamic brain slices at all ages tested and, 2 there is a developmental decrease in Cl-i that is independent of acute trauma caused by brain slicing. We conclude that Cl-i has a developmental progression during P5-20 in both the neocortex and thalamus. However, in both brain regions and during development the neurons closest to the slicing trauma have an elevated Cl-i.



Autor: Joseph Glykys , Kevin J. Staley

Fuente: http://plos.srce.hr/



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