PrPC Undergoes Basal to Apical Transcytosis in Polarized Epithelial MDCK CellsReportar como inadecuado




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The Prion Protein PrP is an ubiquitously expressed glycosylated membrane protein attached to the external leaflet of the plasma membrane via a glycosylphosphatidylinositol anchor GPI. While the misfolded PrPSc scrapie isoform is the infectious agent of prion disease, the cellular isoform PrPC is an enigmatic protein with unclear function. Of interest, PrP localization in polarized MDCK cells is controversial and its mechanism of trafficking is not clear. Here we investigated PrP traffic in MDCK cells polarized on filters and in three-dimensional MDCK cysts, a more physiological model of polarized epithelia. We found that, unlike other GPI-anchored proteins GPI-APs, PrP undergoes basolateral-to-apical transcytosis in fully polarized MDCK cells. Following this event full-length PrP and its cleavage fragments are segregated in different domains of the plasma membrane in polarized cells in both 2D and 3D cultures.



Autor: Alexander Arkhipenko, Sylvie Syan, Guiliana Soraya Victoria, Stéphanie Lebreton , Chiara Zurzolo

Fuente: http://plos.srce.hr/



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