Influence of Age on Ocular Biomechanical Properties in a Canine Glaucoma Model with ADAMTS10 MutationReportar como inadecuado

Influence of Age on Ocular Biomechanical Properties in a Canine Glaucoma Model with ADAMTS10 Mutation - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Soft tissue often displays marked age-associated stiffening. This study aims to investigate how age affects scleral biomechanical properties in a canine glaucoma model with ADAMTS10 mutation, whose extracellular matrix is concomitantly influenced by the mutation and an increased mechanical load from an early age. Biomechanical data was acquired from ADAMTS10-mutant dogs n = 10, 21 to 131 months and normal dogs n = 5, 69 to 113 months. Infusion testing was first performed in the whole globes to measure ocular rigidity. After infusion experiments, the corneas were immediately trephined to prepare scleral shells that were mounted on a pressurization chamber to measure strains in the posterior sclera using an inflation testing protocol. Dynamic viscoelastic mechanical testing was then performed on dissected posterior scleral strips and the data were combined with those reported earlier by our group from the same animal model Palko et al, IOVS 2013. The association between age and scleral biomechanical properties was evaluated using multivariate linear regression. The relationships between scleral properties and the mean and last measured intraocular pressure IOP were also evaluated. Our results showed that age was positively associated with complex modulus p<0.001 and negatively associated with loss tangent p<0.001 in both the affected and the normal groups, suggesting an increased stiffness and decreased mechanical damping with age. The regression slopes were not different between the groups, although the complex modulus was significantly lower in the affected group p = 0.041. The posterior circumferential tangential strain was negatively correlated with complex modulus R = -0.744, p = 0.006 showing consistent mechanical evaluation between the testing methods. Normalized ocular rigidity was negatively correlated with the last IOP in the affected group p = 0.003. Despite a mutation that affects the extracellular matrix and a chronic IOP elevation in the affected dogs, age-associated scleral stiffening and loss of mechanical damping were still prominent and had a similar rate of change as in the normal dogs.

Autor: Joel R. Palko, Hugh J. Morris, Xueliang Pan, Christine D. Harman, Kristin L. Koehl, Kirk N. Gelatt, Caryn E. Plummer, András M.



Documentos relacionados