Correction: Identification of an Extracellular Endoglucanase That Is Required for Full Virulence in Xanthomonas citri subsp. citriReportar como inadecuado




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> Correction: Identification of an Extracellular Endoglucanase That Is Required for Full Virulence in Xanthomonas citri subsp. citri Tian Xia, Yanjiao Li, Dongling Sun, Tao Zhuo, Xiaojing Fan, Huasong Zou x Published: May 23, 2016 https:-doi.org-10.1371-journal.pone.0156458 Article Metrics Comments Related Content Reader Comments 0 Media Coverage Reset zoom 1 { %> All Figures Next Previous show more show less Show in Context

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TIFF PNG PPT S1 Fig is incorrect. Please view the correct S1 Fig below.There are errors in S1 and S2 Tables. The pET30a+ vector of both tables should read pET41a+.Supporting InformationS1 Fig. Ectopic expression of cellulase in E. coli.

Bacteria were cultured in LB medium at 37°C to OD600 0.5. The recombinant proteins were induced for 3 h by supplementation with 0.5 mM IPTG. Cells were harvested, washed in PBS, and then resuspended in 10 mM PBS pH7.5, 500 mM NaCl. After several freeze-thaw cycles, the cell suspension was sonicated for 3 min with an interval of 4 s between pulses, and then centrifuged at 5000 g for 10 min at 37°C. Twenty microliter supernatant samples were analysed by 12% SDS-PAGE.https:-doi.org-10.1371-journal.pone.0156458.s001TIFS1 Table. Strains and plasmids used in this study.

https:-doi.org-10.1371-journal.pone.0156458.s002DOCXS2 Table. Primers used in this study.

https:-doi.org-10.1371-journal.pone.0156458.s003DOCXReference1. Xia T, Li Y, Sun D, Zhuo T, Fan X, Zou H 2016 Identification of an Extracellular Endoglucanase That Is Required for Full Virulence in Xanthomonas citrisubsp. citri. PLoS ONE 113: e0151017. pmid:26950296 View Article PubMed-NCBI Google Scholar



Autor: Tian Xia, Yanjiao Li, Dongling Sun, Tao Zhuo, Xiaojing Fan, Huasong Zou

Fuente: http://plos.srce.hr/



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