Assessment of Free Radical Scavenging Activity of Dimethylglycine Sodium Salt and Its Role in Providing Protection against Lipopolysaccharide-Induced Oxidative Stress in MiceReportar como inadecuado




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In the present study, the free radical scavenging activities against 1,1-diphenyl-2-pierylhydrazy DPPH, 2,2-Azinobis-3-ethylbenzthiazoline-6- sulphonate ABTS+, Hydrogen peroxide H2O2 of dimethylglycine sodium salt DMG-Na were measured and compared with those of Trolox 6-hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid, a commonly used antioxidant. The radical scavenging activities of DMG-Na were found to be the highest at 40 mg-ml. In Experiment 2, gastric intubation in mice with 12 mg DMG-Na-0.3 ml sterile saline solution significantly increased P < 0.05 the body weight BW 28 d, organ proportion liver and spleen, and antioxidant capacity in serum and the liver Superoxide dismutase SOD, Hydrogen peroxidase CAT, Glutathione peroxidase GPx, and Total antioxidant capacity T-AOC, and significantly decreased P < 0.05 the activities of serum Glutamic-pyruvic transaminase ALT and Glutamic oxalacetic transaminase AST and Methane Dicarboxylic Aldehyde MDA contents in the serum and liver. Specifically, the effect of 12 mg DMG-Na-0.3 ml sterile saline solution, which showed the highest antioxidant capacity, was further studied using a mice model. In Experiment 3, the mice CL CON+ lipopolysaccharide LPS group showed a significant decrease P < 0.05 in the serum ALT and AST content; hepatic mitochondrial antioxidant capacity Manganese Superoxide dismutase MnSOD, Glutathione reductase GR, GPx, Glutathione GSH; MDA and Protein carbonyl PC content; Reactive oxygen species ROS level, Mitochondrial membrane potential MMP level, and expression of liver antioxidant genes Nuclear factor erythroid 2-related factor 2 Nrf2, Heme oxygenase 1 HO-1, Manganese superoxide dismutase MnSOD, Glutathione peroxidase 1 Gpx1, Sirtuin 1 Sirt1 relative to the mice CS CON+ sterile saline group. The DL DMG+LPS group showed a significant decrease P < 0.05 in serum ALT and AST content, ROS level, and expression of liver antioxidant gene MnSOD, Gpx1, Sirt1 and a significant increase P < 0.05 in the hepatic mitochondrial antioxidant capacity MnSOD, GSH, GPx, GR and MMP level relative to the CL group. These results indicate that DMG-Na could protect against the LPS-induced oxidative stress by enhancing the free radical scavenging capacity, and increasing the activity of antioxidant defense system.



Autor: Kaiwen Bai , Wen Xu , Jingfei Zhang, Tao Kou, Yu Niu, Xiaoli Wan, Lili Zhang, Chao Wang, Tian Wang

Fuente: http://plos.srce.hr/



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