Effect of Lutein and Antioxidant Supplementation on VEGF Expression, MMP-2 Activity, and Ultrastructural Alterations in Apolipoprotein E-Deficient MouseReportar como inadecuado




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Oxidative Medicine and Cellular LongevityVolume 2013 2013, Article ID 213505, 11 pages

Research Article

Experimental Ophthalmology Laboratory, Clínica Universidad de Navarra, School of Medicine, University of Navarra, ES-31008 Pamplona, Spain

Department of Ophthalmology, Clínica Universidad de Navarra, School of Medicine, University of Navarra, Pio XII 36, ES-31008 Pamplona, Spain

Atherothrombosis Research Laboratory, Division of Cardiovascular Sciences, Center for Applied Medical Research CIMA, University of Navarra, ES-31008 Pamplona, Spain

Received 7 February 2013; Revised 5 April 2013; Accepted 8 April 2013

Academic Editor: Kota V. Ramana

Copyright © 2013 Patricia Fernández-Robredo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Oxidative stress is involved in the pathogenesis of several diseases such as atherosclerosis and age-related macular degeneration AMD. ApoE-deficient mice apoE

are a well-established model of genetic hypercholesterolemia and develop retinal alterations similar to those found in humans with AMD. Thus supplementation with lutein or multivitamin plus lutein and glutathione complex MV could prevent the onset of these alterations. ApoE

mice , 3 months old were treated daily for 3 months with lutein AE-LUT or MV two doses: AE-MV15 15 mg-kg-day and AE-MV50 50 mg-kg-day and were compared to controls with vehicle AE-C. Wild-type mice were also used as control WT-C. ApoE

mice showed higher retinal lipid peroxidation and increased VEGF expression and MMP-2 activity, associated with ultrastructural alterations such as basal laminar deposits, vacuoles, and an increase in Bruch-s membrane thickness. While lutein alone partially prevented the alterations observed in apoE

mice, MV treatment substantially reduced VEGF levels and MMP-2 activity and ameliorated the retinal morphological alterations. These results suggest that oxidative stress in addition to an increased expression and activity of proangiogenic factors could participate in the onset or development of retinal alterations of apoE

mice. Moreover, these changes could be prevented by efficient antioxidant treatments.





Autor: Patricia Fernández-Robredo, Luis M. Sádaba, Angel Salinas-Alamán, Sergio Recalde, José A. Rodríguez, and Alfredo García

Fuente: https://www.hindawi.com/



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